Journal: bioRxiv
Article Title: TMIGD1, a putative tumor suppressor, induces G2-M cell cycle checkpoint arrest in colon cancer cells
doi: 10.1101/2020.06.06.138057
Figure Lengend Snippet: ( A ) Two human colorectal cancer tissue microarray slides consist of 172 tissue samples were stained with anti-TMIGD1 antibody. Staining was scored as 0 (negative, <5% cells positive), 1+ (6-25% cells positive), 2+ (26-50% cells positive), and 3+ (>50% cells positive). The tumor differentiation was graded morphologically (grade 1, 2 and 3 as well, moderately and poorly differentiated). ( B ) The average staining intensities were compared using ANOVA with Tukey post-hoc test per tumor grade. ( C ) The average staining intensities were compared using ANOVA with Tukey post-hoc test per tumor differentiation. ( D ) Western blot analysis of TMIGD1 expression in human renal and colorectal cancer cell lines. ( E ) qPCR analysis of TMIGD1 expression in CRC cell lines (HT29, HCT116 and RKO). Human kidney epithelial cells, HK2 was used as a positive control.
Article Snippet: Two human colorectal cancer tissue microarray slides (US Biomax, catalog numbers BC05012a and BC05118a) consist of 172 tissue samples (72 on BC05012a and 100 on BC05118a) were stained with anti-TMIGD1 antibody.
Techniques: Microarray, Staining, Western Blot, Expressing, Positive Control